Figure 6.

Decreases in cleaved γENaC during Na⁺ repletion of WT and Liddle mice. A. Western blot of kidney microsomes before and 3 or 6 hours after initiation of Na⁺ repletion. Each lane was loaded with 60 μg of protein from a different animal. All mice were male. B. Band density of cleaved γENaC and full-length forms are normalized to values for Na⁺ depleted mice. The ratios of cleaved/full-length are plotted as absolute values. Data represent means ± SEM for n = 4–8 animals. p-values from Student t-tests comparing t = 0 and t = 6 hrs are shown above the bars.