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. 2026 Jan 13;136(5):e196613. doi: 10.1172/JCI196613

Figure 2. Identification of HLA-A*11:01–restricted TP53R248Q-responsive TCR by tetramer staining combined with single-cell TCR sequencing.

Figure 2

(A) Schematic diagram of in vitro stimulation of T cells specific to TP53R248Q, followed by isolation TP53R248Q TCRs by tetramer staining combined with single-cell TCR sequencing. (B) After the third round of in vitro stimulation, CD8+/HLA-A*11:01–TP53R248Q tetramer+ T cells were sorted using flow cytometry. (C) The frequency of top 10 TCR clonotypes was ranked after single-cell TCR sequencing. (D–F) Single-cell transcriptomics analyses the expression of lineage-related, stemness-related, and activation-related markers between the top 3 T cell clones and other T cell clones. (G) In vitro binding assay validation by HLA-A*11:01–TP53R248Q tetramer staining after lentiviral infection with TP53R248Q TCR clones 1, 2, and 3. (H) Flow cytometry identification of the CD8 coreceptor–dependent manner of TP53R248Q TCR clone 2.