Figure 3.
Multidrug-resistant Staphylococcus aureus (MRSA) α-toxin suppresses the killing of MRSA by induced pluripotent stem cell-derived platelets (iPSC-PLTs). (A) Growth curves of wild-type (WT) and α-toxin–deficient (ΔHla)-MRSA measured by changes in the absorbance of optical density (OD)600. (B) MRSA-killing assay of iPSC-PLTs. (C) Flow cytometry analysis of CD62P expression, PAC-1 binding, and annexin V binding in iPSC-PLTs coincubated with WT MRSA or ΔHla-MRSA. Compiled data of 3 independent experiments. Data are presented as means ± SEM. ns, not significant; PLT, platelet.