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. 2026 Feb 2;10(2):103374. doi: 10.1016/j.rpth.2026.103374

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© 2026 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Induced pluripotent stem cell-derived platelets (iPSC-PLTs; Sev2) are activated by Toll-like receptor (TLR) 2 agonists. (A) Flow cytometry analysis of TLR2, TLR4, and TLR9 expression on SeV2. (B–D) Flow cytometry analysis of CD62P expression, PAC-1 binding, and annexin V binding in Sev2 following stimulation with increasing concentrations of Pam3CSK4 or peptidoglycan. (E) Flow cytometry analysis of intracellular calcium levels in Sev2 following stimulation with a TLR2 agonist (Pam3CSK4 or peptidoglycan). (F) Long-term measurements of intracellular calcium concentration in Sev2 stimulated with a TLR2 agonist (Pam3CSK4 or peptidoglycan). Values are relative to levels in the presence of ethylene glycol tetraacetic acid (EGTA). Compiled data of 3 independent experiments. Data are presented as means ± SEM. ∗P < .05, ∗∗P < .01, ∗∗∗∗P < .0001. MFI, mean fluorescence intensity.