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MS2 spectrum and the cognate neutral loss MS3 spectrum of the phosphopeptide LGYTGENGGGQSEpSPVKDETPR exemplifying the additional sequence information gained of an MS2 spectrum compared to a MS3 spectrum. A: MS2 spectrum (Xcorr: 2.424); B: MS3 spectrum (Xcorr: 3.248). Exact localisation of the phosphorylation site is only possible with the MS3 spectrum in this case. Only ions above the noise signal were annotated.
