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. 2026 Feb 27;83(1):134. doi: 10.1007/s00018-025-06069-x

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© The Author(s) 2026

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — m⁶A methylation participate in regulation of mRNAs expression level in olfactory bulb. (A) Cumulative frequency plots showing the correlation between methylation and mRNA expression level between pairwise comparisons. (B) Nine quadrant graphs showing the DEGs containing differential methylation. (C) Cumulative frequency plots showing how methylation status correlates with mRNA expression levels among three conditions. (D) Nine quadrant graphs exhibiting the DEGs containing differently methylations. (E) MeRip-qPCR assay indicating the m⁶A methylation levels of Wnt pathway genes Wnt4, Ror2 and Nkd1analyzed, as determined by Student’s t-test. (n = 3 per group) (F) RT- qPCR assay indicating the mRNA expression level of Wnt4, Ror2 and Nkd1, as determined by Student’s t-test. (n = 3 per group)