Fig. 6.

Dynamic changes of m⁶A enzyme in the olfactory bulb at different stages. (A) Immunohistochemistry of olfactory bulb at three conditions using antibodies against the METTL3.(n = 5 per group) (B) Quantification of METTL3 IOD/Area of positive cells, as determined by ANOVA. (C) Immunohistochemistry of olfactory bulb at three conditions using antibodies against the FTO. (n = 5 per group) (D) Quantification of FTO IOD/Area of positive cells, as determined by ANOVA. (E) Immunohistochemistry of olfactory bulb at three conditions using antibodies against the YTHDF2.(n = 5 per group) (F) Quantification of YTHDF2 IOD/Area of positive cells, as determined by ANOVA. (G) RT-qPCR assay indicating the mRNA expression level of Mettl3, Fto and Ythdf2, as determined by Student’s t-test.(n = 3 per group) (H) The correlation of expression level between m⁶A enzyme and Wnt pathway involved genes