Fig. 7.

Mettl3 knockdown rescue the age-related olfactory dysfunction. (A) The flowchart of intervention experiment. (B) GFP (red) immunostaining in old mouse injected with lentivirus after 10 days. (C) Representative immunohistochemistry of shMettl3-injected mice and control mice using antibodies against the METTL3. (D) Quantification of METTL3 IOD/Area of positive cells, as determined by Student’s t-test. (E) Box plot showing the latency time of shMettl3 and control group in buried food seek buried test, as determined by Student’s t-test. (n = 5 per group) (F) Box plot showing the avoid index of shMettl3 and control group in olfactory avoidance test, as determined by Student’s t-test. (n = 5 per group) (G) The investigation time of shMettl3 and control group in olfactory habituation-dishabituation test, as determined by Student’s t-test. (n = 5 per group) (H) Box plot showing the weight of shMettl3 and control group, as determined by Student’s t-test. (I) RT-qPCR assay indicating the mRNA expression level of Wnt4, Ror2 and Nkd1 in shMettl3 and control group, as determined by Student’s t-test.(n = 3 per group) (J) Representative immunohistochemistry of shMettl3-injected mice and control mice using antibodies against the VGLUT1. (n = 5 per group) K) Quantification of VGLUT1 IOD/Area of positive cells, as determined by Student’s t-test