Fig. 1: morphology of Trypanosoma cruzi epimastigotes in prolonged starvation cell culture. Representative images of parasites observed by Confocal microscopy Leica TCS-SP5 100X. Top and bottom panels: epimastigote (D7) and metacyclic trypomastigote-like parasite (M) forms observed in exponentially growing epimastigote cultures. Middle panels: intermediate forms observed in starved cell cultures at days: 14, 21 and 28 (D14, D21 and D28 respectively). Right panel: phase contrast, central panel: DAPI stained parasites, left panel: overlay of both images.
