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. 2026 Feb 23;17:1707203. doi: 10.3389/fimmu.2026.1707203

Figure 6.

Figure with three panels analyzing cellular responses under various conditions. Panel A shows two line graphs depicting optical density over 24 hours for different treatments, with notable differences marked by asterisks. Panel B includes flow cytometry plots for different conditions, each labeled with the proportion of cells in various phases. Panel C displays Western blot images and a corresponding bar graph comparing protein expression levels across conditions. Statistical significance is indicated by asterisks in all panels.

SLC31A1 silencing hampers cardiomyocyte apoptosis via the NLRP3/HMGB1-dependent macrophage cuproptosis pathway. (A) H9C2 cell viability assessed by CCK-8; (B) H9C2 cell apoptosis analyzed by flow cytometry; (C) Cleaved caspase-3, Bcl-2 and Bax protein levels in H9C2 cells determined by western blot. All cell-based in vitro experiments were independently repeated three times. Data were described as mean ± standard deviation, with multi-group comparisons conducted by one-way ANOVA, followed by Tukey’s post hoc tests. *p < 0.05, **p < 0.01, ***p < 0.001.