Table 2.
Summary of crystallographic data: Ep mutants
| Crystal | L89T–TTP | Y177F–UDG | W224H–UDG |
|---|---|---|---|
| Resolution, Å | 2.2 | 2.75 | 2.2 |
| Wavelength, Å | 1.072 | 1.54 | 1.14 |
| Completeness, % | 95.0 | 98.3 | 94.3 |
| Redundancy, fold | 4 | 5 | 17.5 |
| I/σI | 24.3 | 18.2 | 40.1 |
| Rmerge | 4.9 | 6.1 | 6.1 |
| Space Group | P43212 | P212121 | P6522 |
| Cell Dimensions, Å | a = b = 120, c = 94 | a = 91, b = 111, c = 131 | a = b = 88, c = 329 |
| Refinement | |||
| Resolution, Å | 8–2.2 | 8–2.75 | 8–2.2 |
| Reflections working/test | 29,105/3,206 | 30,716/3,486 | 34,900/3,911 |
| Nonhydrogen atoms | 4,626 | 9,172 | 4,580 |
| Rcrys/Rfree | 24.3/28.1 | 22.9/29.2 | 25.7/28.9 |
| rms deviations | |||
| Bonds, Å | 0.008 | 0.009 | 0.007 |
| Angles, ° | 1.30 | 1.34 | 1.26 |
Rsym = Σ|I − 〈I〉|/ΣI, where I = observed intensity and 〈I〉 = average intensity obtained from multiple observations of symmetry related reflections. rms bond lengths and rms bond angles are the respective root-mean-square deviations from ideal values. Rcrys and Rfree are the respective R factor and free R factor of the crystal (using 10% of the data as the test set).