Figure 2.

Deletion of HCF-1_AD does not affect association with the N terminus or nuclear localization. (A) HA-tagged HCF-1_N450–1011 and T7-tagged HCF-1_C WT or ΔAD were coexpressed in transfected 293T cells and association assayed by immunoprecipitation with αHA antibody followed by immunoblotting with the αT7 antibody. The HA-tagged HCF-1_N450–1011 is in lanes 1–3 and the T7-tagged HCF-1_C WT and ΔAD in lanes 1 and 4 and lanes 2 and 5, respectively. (B) HeLa cells were transfected with expression plasmids (500 ng) encoding GFP or GFP-tagged HCF-1_C WT or ΔAD. DNA is visualized with Hoechst 33258 and GFP by fluorescence microscopy.