Figure 7.

Purification of rhodopsin mutant E113Q/E134Q/M257Y from the HEK293S GnTI⁻ stable cell line. Column profile showing A₂₈₀ and A₅₀₀. Fractions (1.5 ml) were collected and washes were E1 (buffer C), E2 (buffer D, pH 7.2), E3 as E2 + 100 μM 9-mer rhodopsin elution peptide, and E4 as E1 + 100 μM elution peptide. (Inset) SDS/PAGE (10%) analysis of selected column fractions with detection either by silver stain or by immunoaffinity with rho-1D4. S, solubilized cell lysate; F, column flowthrough; M, molecular weight markers.