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. 2002 Oct 7;99(21):13498–13503. doi: 10.1073/pnas.212474399

Figure 4.

Figure 4

Genotyping of ARKO mice. We have applied the primers “select” and “2–9” to identify wt and ARKO male mice in our study. (A) Schematic presentation of the DNA construct and primer location in exon 2 area of wt, KO, and floxed AR genes. (B) List of the sizes of PCR product amplified by designed primer pairs. (C) The identification of wt and ARKO mice, by using select and 2–9. We amplified a DNA fragment with 580 bp, which represents wtAR, and with 238 bp, which represents ARKO exon 2. The expression of Cre and internal control IL-2 were confirmed by PCR (Bottom).