Table 1.
Phase transitions in lipid membranes in the presence of α-crystallin and HSP17 (molar ratio lipid/protein in parentheses)
| Lipid | Pretransition, °C | Main transition, °C | Nonlamellar transition, °C |
|---|---|---|---|
| DMPG in buffer | 18.0 ± 0.2 | 28.0 ± 0.3 | |
| DMPG/αC (55:1) | 17.0 ± 0.3 | 24.3 ± 0.4 | |
| DMPG/HSP17 (55:1) | 16.6 ± 0.3 | 24.2 ± 0.3 | |
| DMPS in buffer | 39.6 ± 0.2 | ||
| DMPS/αC (55:1) | 38.0 ± 0.4 | ||
| DMPS/HSP17 (55:1) | 37.4 ± 0.3 | ||
| DEPE in buffer | 38.1 ± 0.2 | 61.3 ± 0.4 | |
| DEPE/HSP17 (110:1) | 38.0 ± 0.3 | 64.4 ± 0.5 | |
| DEPE/HSP17 (55:1) | 37.6 ± 0.2 | 67.8 ± 0.6 | |
| DEPE/αC (110:1) | 38.0 ± 0.3 | 63.8 ± 0.6 | |
| DEPE/αC (55:1) | 37.6 ± 0.4 | 67.1 ± 0.6 | |
| MGDG in buffer | 29.7 ± 0.3 | ||
| MGDG/αC (55:1) | 30.8 ± 0.3 | ||
| MGDG/HSP17 (55:1) | 32.0 ± 0.2 |
Data are derived from DSC studies and are means of at least three measurements in three independent preparations.