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Effect of F1395 mutations on nicotinamide coenzyme utilization by nNOS
| Enzyme | NADPH
|
NADH
|
||
|---|---|---|---|---|
| Ferricyanide reduction, min−1 | Cytochrome c reduction, min−1 | Ferricyanide reduction, min−1 | Cytochrome c reduction, min−1 | |
| nNOS | 5,052 ± 500 | 7,000 ± 400 | 92 ± 3 | 60 ± 3 |
| F1395Y | 6,059 ± 500 | 6,090 ± 250 | 110 ± 5 | 56 ± 2 |
| F1395S | 5,550 ± 440 | 2,025 ± 200 | 2,851 ± 200 | 1,348 ± 40 |
