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Kinetic comparison of cytochrome c reductase activity supported by NADPH or NADH
| Enzyme | NADPH
|
NADH
|
NADPH/NADH | ||||
|---|---|---|---|---|---|---|---|
| Km, μM | Vmax, min−1 | Vmax/Km (A), μM−1⋅min−1 | Km, μM | Vmax, min−1 | Vmax/Km (B), μM−1⋅min−1 | (A/B) | |
| nNOS | 4.8 ± 0.2 | 7,952 ± 700 | 1,656 | 5,000 ± 500 | 1,600 ± 100 | 0.32 | 4,987 |
| F1395Y | 4.0 ± 0.2 | 6,740 ± 650 | 1,685 | 4,400 ± 600 | 1,660 ± 50 | 0.377 | 4,469 |
| F1395S | 1.9 ± 0.1 | 2,726 ± 300 | 1,434 | 250 ± 15 | 3,464 ± 150 | 13.86 | 103 |
