Figure 3.

Assembly of transcription factor complex at a megakaryocyte-specific gene. A schematic representation of the proximal promoter region of a hypothetical megakaryocyte-specific gene modeled after the Itga2b gene. GATA-1 is shown in the middle binding to its known consensus sequence (69) and interacting with FOG-1, Fli-1, and CBFA2 of the RUNX complex (each interaction is indicated by a 2-headed arrow). Fli-1 and CBFA2 bind to their adjacent cognate on the DNA (89, 122), while FOG-1 is recruited through its interactions with the N-terminal zinc finger of GATA-1. In turn, FOG-1 recruits the NuRD complex and other nuclear factors.