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. 2002 Oct 7;99(21):13647–13652. doi: 10.1073/pnas.212516099

Figure 2.

Figure 2

Deletion analysis of tumor samples. (A) Real-time PCR analysis of a breast tumor sample CHTN41 is demonstrated. The abscissa is cycle number of PCR and the ordinate is quantity of PCR products. The control primer set in this figure is WB23 from chromosome 22. “D” and “A” represent DNA from diploid and aneuploid fractions, respectively. The amplification curve from aneuloid DNA with WD31 primer set has a several cycle difference from the others, indicating deletion of WD31 in this tumor. (B) Southern blot analysis of DpnII representation with WD31 probe is shown. Each lane contains 5 μg of DpnII restriction fragments. Lanes 1 and 2 are DNA from a tumor and a matched normal (CHTN40), respectively, showing no deletion. Lanes 3 and 4 contain DNA from a tumor and a matched normal pair (CHTN41), showing deletion. ER48 is a control probe derived from chromosome 3. Lane 3 has a very faint band for DBC2, whereas the control probe exhibits the same intensity for tumor and normal samples, representing homozygous deletion. Contamination of normal stromal cells is considered to contribute the faint band.