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Immunoelectron microscopy showing localization of SIRT3–EGFP to the mitochondrial cristae. (A) Indirect immunogold labeling of ultrathin cryosections of human cervical carcinoma (SiHa) cells demonstrates SIRT3–EGFP label (5 nm gold) in plain association with mitochondria in three independent images (A–C). High-magnification images reveal label clearly associated with mitochondrial cristae (B and C). m, mitochondria; arrowheads, cristae labeling. (Bars, 0.5 μm.)
