Figure 3.
In vitro and ex vivo translation of the G72 candidate gene. (a) Coupled in vitro transcription/translation assays in the presence of [35S]-Met for both LG72 (lane 1) and LG30 (lane 2) cDNAs, unprimed lysates (lane 3), and a luciferase cDNA control (lane 4). (b) Expression vectors harboring the nontagged LG72 cDNA either in the antisense orientation (−) (lane 2) or in the sense orientation (+) (lane 3) were transfected into COS-7 cells and the expressed proteins analyzed with a purified anti-pLG72 serum. A His-Xpress-pLG72 recombinant protein (5 ng) purified from bacteria was run as a control (time exposure for this lane is shorter than for the two others). The His-Xpress tag is 33 amino acids long.