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. 2005 Oct 4;2(4):529–536. doi: 10.1093/ecam/neh123

Table 1.

Pairs of synthetic primers used in RT–PCR

Oligonuleotides Sequence Expected size
IL-12 5′-primer 5′-ACCTCAGTTTGGCCAGGGTC-3′ 500
3′-primer 5′-GTCACGACGCGGGTGGTGAAG-3′
IFN-γ 5′-primer 5′-TACTGCCACGGCACAGTCATTGAA-3′ 405
3′-primer 5′-GCAGCGACTCCTTTTCCGCTTCCT-3′
IL-1β 5′-primer 5′-TGAAGGGCTGCTTCCAAACCTTTGACC-3′ 361
3′-primer 5′-TGTCCATTGAGGTGGAGAGCTTTCAGC-3′
TNF-α 5′-primer 5′-GCGACGTGGAACTGGCAGAAG-3′ 340
3′-primer 5′-TCCATGCCGTTGGCCAGGAGG-3′
Mn-SOD 5′-primer 5′-GCACATTAACGCGCAGATCA-3′ 241
3′-primer 5′-AGCCTCCAGCAACTCTCCTT-3′
Cu/Zn-SOD 5′-primer 5′-AAGGCCGTGTGCGTGCTGAA-3′ 246
3′-primer 5′-CAGGTCTCCAACATGCCTCT-3′
Catalase 5′-primer 5′-GCAGATACCTGTGAACTGTC-3′ 229
3′-primer 5′-GTAGAATGTCCGCACCTGAG-3′
GPx 5′-primer 5′-CCTCAAGTACGTCCGACCTG-3′ 197
3′-primer 5′-GTAGAATGTCCGCACCTGAG-3′
HO-1 5′-primer 5′-AACAAGCAGAACCCAGTC-3′ 375
3′-primer 5′-TGTCATCTCCAGAGTGTTC-3′
TGF-β1 5′-primer 5′-GCTCACTGCTCTTGTGACAGCAAAG-3′ 360
3′-primer 5′-CAAGGACCTTGCTGTACTGTGTGTC-3′
β-actin 5′-primer 5′-TGGAATCCTGTGGCATCCATGAAAC-3′ 349
3′-primer 5′-TAAAACGCAGCTCAGTAACAGTCCG-3′