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Osteoblast differentiation is not altered in TRPV5-/- mice. Murine bone marrow cells from TRPV5+/+ and TRPV5-/- mice were either cultured for 10 days and stained for ALP or cultured for 20 days and stained for mineralization (alizarin red). The number of ALP- and alizarin red-positive colonies were quantified. Values are presented as mean ± SD.
