Table 1. Sequences of primers and Taqman probes for real-time PCR.

Gene	Species	Forward primer	Reverse primer	Probe
HPRT	M	TTATCAGACTGAAGAGCTACTGTAATGATC	TTACCAGTGTCAATTATATCTTCAACAATC	TGAGAGATCATCTCCACCAATAACTTTTATGTCCC
GAPDH	H	ATGGGGAAGGTGAAGGTCG	TAAAAGCAGCCCTGGTGACC	CGCCCAATACGACCAAATCCGTTGAC
TRPV5	M	CGTTGGTTCTTACGGGTTGAAC	GTTTGGAGAACCACAGAGCCTCTA	TGTTTCTCAGATAGCTGCTCTTGTACTTCCTCTTTGT
	H	TTGCCTCCGCGTTCTATATCA	GGCAAGTCCACGTCGTAGTTG	ACTACCCCATGGCACTGTTCACCACC
CaBP-D9k	M	CCTGCAGAAATGAAGAGCATTTT	CTCCATCGCCATTCTTATCCA	CAAAAATATGCAGCCAAGGAAGGCGA
	H	AATGAGTACTAAAAGTCTCCTGAGGAACT	AGGGTGTTTGGACCTTTGAGTAAA	TCTGGATCACCTTCTTTGGCTGCATATTTTTC
CaBP-D28k	M	AACTGACAGAGATGGCCAGGTTA	TGAACTCTTTCCCACACATTTTGAT	ACCAGTGCAGGAAAATTTCCTTCTTAAATTCCA
	H	CCTAATGCTGAAACTATTTGATTCAAATAA	TCTTTCCCACACATTTTGATTCC	CCAGGTTACTACCAGTGCAGGAGAATTTTCTTCTTAA
NCX1	M	TCCCTACAAAACTATTGAAGGCACA	TTTCTCATACTCCTCGTCATCGATT	ACCTTGACTGATATTGTTTTGACTATTTCATCATTCTGGA
	H	CAAAACAATATCAGTCAAGGTAATTGATG	CCTCACGGTCAAATATTCTAATGGT	AAGACCTTCTTCCTTGAGATTGGAGAGCCC
PMCA1	M	CGCCATCTTCTGCACCATT	CAGCCATTGCTCTATTGAAAGTTC	CAGCTGAAAGGCTTCCCGCCAAA
	H	CAACAATTCCAACTAGCCGTTTAA	GGCCACGCCGCAACT	CCTTTTGTGTTCCATGACCAGCTTCTTTGA

PCR primers and fluorescent probes (5′-6-carboxyfluorescein- and 3′-6-carboxytetramethylrhodamin-labeled) were designed by using the computer program primer express (version 1.5; Applied Biosystems) and were purchased from Applied Biosystems (GAPDH), Eurogentec (other human sequences), or Biolegio (Malden, The Netherlands) (mouse sequences). HPRT, hypoxantine-guanine phosphoribosyl transferase; TRPV5, transient receptor potential channel V5; CaBP, calbindin; M, murine; H, human.