Table 2. Cytokine microarray analyses in the supernatants of confluent cells.
| Normalized cytokine production
|
||||
|---|---|---|---|---|
| Growth factors | NK1-Tr transfectants | NK1-FL transfectants | BCC | Nontumorigenic breast cells |
| Chemokines | ||||
| RANTES, GRO-α | 9.8 ± 0.1 | 0.12 ± 0.1 | 9.5 ± 0.03 | 0.1 ± 0.01 |
| MCP-1, IL-8 | 8.1 ± 0.01 | 0.2 ± 0.12 | 8.0 ± 0.02 | 0.1 ± 0.001 |
| MIP-1β | 5.2 ± 1 | 0.15 ± 0.01 | 4.5 ± 0.15 | 0.1 ± 0.01 |
| SDF-1α | 8.2 ± 0.2 | 0.2 ± 0.12 | 8.0 ± 0.25 | ND |
| Angiogenin | 10 ± 0.4 | 0.1 ± 0.02 | 9.8 ± 0.1 | 0.1 ± 0.022 |
| Oncostatin M | 10 ± 0.3 | 0.15 ± 0.01 | 9.5 ± 0.13 | 0.1 ± 0.012 |
| Cytokines | ||||
| IL-6 | 10 ± 0.5 | 5.5 ± 0.02 | 10 ± 0.2 | 3.0 ± 0.2 |
| IL-2 | 10 ± 0.3 | 5 ± 0.3 | ND | ND |
| IL-12 | ND | ND | 0.1 ± 0.01 | ND |
| IFN-α, TNF-α, IL-1α | 10 ± 0.4 | 1.5 ± 0.2 | 10 ± 0.5 | ND |
| IL-4, IL-10, IL-13* | 8 ± 1.2 | 1.5 ± 12 | 9 ± 1.5 | ND |
| TGF-β* | 10 ± 1.5 | 2.2 ± 0.14 | 10 ± 0.3 | ND |
| G-CSF, GM-CSF† | 10 ± 1.1 | 1.8 ± 0.04 | 10 ± 0.5 | ND |
Cell-free media were analyzed from breast cancer cell lines (n = 5). Two different cell passages of two nontumorigenic cells stably transfected with NK1-Tr or NK1-FL cDNA expression plasmid (n = 4) or untransfected (n = 4). The densitometric scans of the internal positive controls were normalized to 10, and the unknowns were calculated accordingly and presented as the mean ± SD. ND, none detected; *, antiinflammatory; †, hematopoietic growth factors.