Table 2. Regression statistics of extracted cell cake water versus wash water.
δ18O of growth medium water, ‰ | Slope of extracted cell water versus wash water | R2 of regression | y intercept value† | Calculated δ18O of intracellular water, ‰*† |
---|---|---|---|---|
-15.2 | 0.81 | 0.99 | -1.2 | -6.32 |
-4.9 | 0.85 | 0.99 | -0.77 | -5.13 |
5.5 | 0.93 | 0.99 | -0.04 | -0.55 |
16.1 | 0.86 | 0.99 | +0.32 | 2.26 |
Average = 0.86‡ | ||||
Standard error = 0.025 |
The δ18O value of intracellular water = (y intercept)/(1 - slope). Intracellular water is itself a combination of growth medium water and intracellular water as described by Eq. 3.
Average and standard error are not presented, because the y intercept values and the δ18O values of the intracellular water are expected to be different as a result of the different δ18O values of the growth medium waters.
These slopes are not statistically different (F = 1.59; F0.05 = 4.35) (13).