Table 1. Gene-specific primers for human GHRH, pGHRH-R, Svs, and β-actin used in this study.

	Sense primers			Antisense primers
Gene	Sequence	cDNA location	Nucleotide location	Sequence	cDNA location	Nucleotide location	Product size, bp
SV1	tggggagagggaaggagttgt	Intron 3	287-307	gcgagaaccagccaccagaa	Exon 7	790-809	523
SV2	aggaaggccccatagtgtgtc	Intron 3	145-165	ggcagccagtggagaagct	Exon 6/8	652-670	526
SV3	gccccatagggctgtgaaac	Intron 3	151-170	acagctgggtgtggacgtagt	Exon 6	375-395	245
SV4	ctgaggagggctgcccgt	Exon 4/8	425-442	ggccctttgatgatccaccagt	Exon 8	523-544	120
pGHRH-R	atgggctgctgtgctggccaac	Exon 3	230-251	taaggtggaaagggctcagacc	Exon 4	353-374	145
GHRH	atgcagatgccatcttcaccaa	—	95-116	tgctgtctacctgacgaccaa	—	224-244	150
β-actin	ctggaacggtgaaggtgaca	—	1348-1367	aagggacttcctgtaacaatg	—	1467-1487	140

β-actin was used as housekeeping gene. —, not applicable.