Table 2. Real-time PCR analysis for GHRH SV receptors in normal human tissues and human cancer lines grown in nude mice.

	CT values, mean ± SEM					Ratio
Samples	SV1	SV2	SV3	SV4	β-actin	SV1	SV2	SV3	SV4
HT	25.93 ± 0.41	28.80 ± 0.74	38.05 ± 0.85	32.98 ± 2.15	13.50 ± 0.35	2.91	45.27	0.78	1.30
RL	26.28 ± 0.80	30.30 ± 0.94	38.90 ± 0.30	34.58 ± 0.91	12.58 ± 0.12	1.22	8.84	0.23	0.23
MiaPaCa-2	26.86 ± 0.60	32.18 ± 0.94	—	—	12.10 ± 0.19	0.58	1.80	—	—
H82	25.55 ± 0.11	—	41.25 ± 2.35	35.35 ± 0.60	12.95 ± 0.27	2.64	—	0.06	0.18
H345	—	—	—	35.05 ± 0.56	12.45 ± 0.37	—	—	—	0.15
DBTRG-05	25.40 ± 0.46	32.30 ± 0.39	35.65 ± 0.65	31.25 ± 0.72	11.35 ± 0.20	1.00	1.00	1.00	1.00
Prostate	26.65 ± 0.15	30.80 ± 0.40	36.40 ± 0.09	31.60 ± 0.22	16.60 ± 0.01	0.19	0.063	0.01	0.0011
Kidney	30.60 ± 0.09	32.35 ± 0.15	40.70 ± 0.80	31.93 ± 0.35	16.15 ± 0.11	0.01	0.016	0.00041	0.0007
Lung	27.75 ± 0.25	30.55 ± 0.05	40.20 ± 0.50	31.80 ± 0.10	15.45 ± 0.11	0.04	0.034	0.00036	0.0005
Liver	29.20 ± 0.30	33.40 ± 0.09	41.85 ± 1.35	34.60 ± 0.60	17.65 ± 0.03	0.06	0.022	0.00050	0.0003
Pituitary	26.70 ± 1.30	29.25 ± 0.25	32.50 ± 0.30	24.23 ± 0.12	19.15 ± 0.03	1.00	1.00	1.00	1.00

The relative gene expression ratio values were calculated based on ref. 24. —, not detectable.