Table 3. Real-time PCR analysis for GHRH and pGHRH-R in normal human tissues and human cancer lines grown in nude mice.

	CT values, mean ± SEM			Ratio
Samples	pGHRH-R	GHRH	β-actin	pGHRH-R	GHRH
HT	32.05 ± 1.75	36.40 ± 1.05	13.50 ± 0.35	0.478	0.60
RL	32.38 ± 0.14	31.85 ± 0.29	12.58 ± 0.12	0.204	7.48
MiaPaCa-2	37.75 ± 0.88	35.83 ± 0.44	12.10 ± 0.19	0.003	0.35
H82	34.82 ± 0.35	33.05 ± 0.09	12.95 ± 0.27	0.045	4.19
H345	33.80 ± 0.16	19.33 ± 0.08	12.45 ± 0.37	0.085	37817.6
DBTRG-05	29.00 ± 0.62	33.58 ± 0.22	11.35 ± 0.20	1	1
Prostate	38.47 ± 1.04	37.46 ± 0.49	16.60 ± 0.01	0.000003	0.044
Kidney	30.93 ± 0.09	36.80 ± 0.90	16.15 ± 0.11	0.000472	0.051
Lung	33.63 ± 0.04	37.38 ± 0.23	15.45 ± 0.11	0.000043	0.021
Liver	37.43 ± 1.19	38.40 ± 0.21	17.65 ± 0.03	0.000012	0.046
Pituitary	23.00 ± 0.08	35.40 ± 0.40	19.15 ± 0.03	1	1

The relative gene expression ratio values were calculated based on ref. 24.