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Modified RT-ROL assay. In this assay antisense binding sites are identified by the ability of a random 9mer oligodeoxynucleotide library (ROL) terminating a specific base and a PCR tag to prime complementary DNA (cDNA) synthesis by reverse transcriptase (RT). The cDNA is then amplified by PCR with a radiolabeled primer having the same sense as the RNA, and an unlabelled primer having the same sequence as the PCR tag.
