Table 1.

PCR conditions^a

Name	Step 1		Step 2		Step 3		Run time (min)
	°C	s	°C	s	°C	s
Conventionalb	95	15	60	15	72	60	60
Universal	95	15	60	60	n/ac	n/a	50
Afast	95	1	64	35	n/a	n/a	24
Mfast	95	5	60	15	72	15	23
Sfast	95	10	60/62	30	n/a	n/a	26

^aAll protocols are preceded by an initial 5 min at 95°C step to activate the polymerase and repeated for 40 cycles.

^bConventional conditions are shown for comparison assuming an extension time of 60 s per 1000 bp amplicon length and optimal Taq polymerase activity at 72°C (7).

^cNon applicable.