Table 2.

The PCR system parameter setting

DNA fragment	Denaturation	Annealing	Extension
Real-time PCR (LightCycler, Roche) detection point 83°C, 1 s
EGFP-I (173 bp)	95°C, 5 s	58°C, 5 s	72°C, 10 s
PT4K2B(752 bp)	95°C, 5 s	55°C, 5 s	72°C, 30 s
MS1R (1236 bp)	95°C, 15 s	53°C, 15 s	72°C, 40 s
BNIP3 (238 bp)	95°C, 10 s	60°C, 5 s	72°C, 12 s
BNIP3 tissue	95°C, 10 s	60°C, 5 s	72°C, 10 s
Conventional PCR (ABI 9600; Applied Biosystem Launched)
EGFP-I (173 bp)	95°C, 5 s	58°C, 5 s	72°C, 10 s
PT4K2B (752 bp)	95°C, 5 s	55°C, 5 s	72°C, 30 s
MS1R (1236 bp)	95°C, 15 s	53°C, 15 s	72°C, 40 s