Figure 2.

RT-PCR analysis of Salmonella LSMMG-regulated genes. The expression of Salmonella genes identified by microarray analysis was analyzed using RT-PCR with total RNA from LSMMG and 1 × g HARV cultures as described in Materials and Methods. The ratio of the normalized quantitated signals is presented as the LSMMG value divided by 1 × g value. The DNA sequences of the primers used are published as supporting information on the PNAS web site. The prgI and ssaK genes are adjacent to other LSMMG-regulated genes in their respective operons. (A) LSMMG up-regulated genes. (B) LSMMG down-regulated genes.