Figure 2.

Flow cytometry of the mTIGIT receptor. A, HeLa cells transfected with a plasmid expressing the mTIGIT receptor were stained with PE-TIGIT (light blue) or PE-IgG (red). B, Quantitative flow on transfected TIGIT-expressing cells shows an average of 9.5 × 10⁴ receptors per cell. C, Live cell binding assay for the determination of binding specificity of TIGITmAb and DFO-TIGITmAb on activated mouse splenocytes. Splenocytes were activated with PMA and calcium ionomycin (50:500 ng/mL for 4 hours). Cells (1 × 10⁶) were stained with isotype control mouse IgG, TIGIT-mAb or DFO-TIGITmAb, followed by anti-mouse-IgG APC staining. Histogram traces correspond to induced splenocytes stained with the following mAbs (1 μg/0.1 mL): mouse IgG isotype control (open histogram), DFO-TIGITmAb (dotted histogram), and unmodified TIGITmAb (filled histogram) for TIGIT expression.