Figure 4.

PodJ_L and PodJ_S steady-state levels peak at different times during the cell cycle. Swarmer cells were isolated by Ludox gradient centrifugation and released into M2G medium. Samples of equal volume were collected from the synchronous culture every 20 min and subjected to immunoblotting by using the PodJ NTD and CTD antibodies, with antibodies to CtrA as a control. (Top) The subcellular location of PodJ_L (red) and PodJ_S (green) during the cell cycle inferred from the results shown below and those shown in Fig. 5. (Middle) The relative levels of PodJ_L (red) and PodJ_S (green) steady-state levels during the cell cycle, determined from the immunoblots shown (Bottom).