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. 2002 Oct 4;99(21):13843–13848. doi: 10.1073/pnas.212400099

Figure 4.

Figure 4

Real-time PCR analysis of HIV-1 and SIV reverse transcripts synthesized shortly after infection. Human and simian cells were infected with NL4.3R−(VSV-G) at moi 0.1, 1.0, and 10. Genomic DNA was prepared at the indicated time points, and reverse transcripts were quantitated by real-time PCR by using primers that detected (A) late reverse transcripts, (B) 2-LTR circles (2-LTR circles were measured at moi 10). Infection with SIVmac239(VSV-G) (C) and SIVagmSAB-1(VSV-G) (D). The data are plotted as copy numbers/500 ng of DNA. Cultures pretreated with nevirapine had 40 copies/500 ng of DNA and did not increase over time (not shown).