Table 1. gRNA-marker collection.
| Chr Arm | Target site | gRNA location | pMAGIC vector | nMAGIC vector | Clone frequency | BDSC IDs ‡ |
|---|---|---|---|---|---|---|
| 2L | 40D2 | attP[VK00037] | pAC-U63-gRNA2.1-ubiGal80(DE)-His2Av | pAC-U63-tgRNA-nlsBFP* pAC-U63-gRNA2.1-tub-miRFP680-T2A-HO1(HA) (HA) | 31 | 606005 and 92741 |
| 2L | 40D4 | attP[VK00037] | pAC-U63-tgRNA-Gal80 * | pAC-U63-tgRNA-nlsBFP * | 13 | 92744 and 92742 |
| 2L | 40E1 | attP[VK00037] | pAC-U63-tgRNA-Gal80 * | pAC-U63-tgRNA-nlsBFP * | 4 | 606004 and 606003 |
| 2R | 41F9 | attP[VK00018] | pAC-U63-gRNA2.1-ubiGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 10 | 606006 and 606010 |
| 2R | 41F11 | attP[VK00018] | pAC-U63-gRNA2.1-ubiGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 14 | 606007 and 606009 |
| 2R | 42A4 | attP[VK00018] | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 14 | 606008 and 606011 |
| 3L | 80F5 | attP2 | pAC-U63-gRNA2.1-tubGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 11 | 606014 and 606017 |
| 3L | 80C2 | attP2 | pAC-U63-gRNA2.1-tubGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 7 | 606013 and 606016 |
| 3L | 80C1 | attP2 | pAC-U63-gRNA2.1-tubGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 4 | 606012 and 606015 |
| 3R | 81 F | attP[VK00027] | pAC-U63-gRNA2.1-tubGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 4 | 606020 and 606021 |
| 3R | 82A4 | attP[VK00027] | pAC-U63-gRNA2.1-tubGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 10 | 606018 and 606022 |
| 3R | 82C3 | attP[VK00027] | pAC-U63-gRNA2.1-tubGal80(DE)-His2Av | pAC-U63-gRNA2.1-tubBFP(HA) | 16 | 606019 and 606023 |
| X | X2 | attP18 | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 19 | 606024 and 606028 |
| X | 20F1 | attP18 | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 18 | 606025 and 606779 |
| X | 20F2 | attP18 | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 19 | 606026 and 606029 |
| IV | 101F1-a | attP[ZH-102D]† | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 4 | 606776 and 606780 |
| IV | 101F1-b | attP[ZH-102D]† | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 25 | 606777 and 606781 |
| IV | 101F1-c | attP[ZH-102D]† | pAC-U63-gRNA2.1-tubGal80(DE)-SV40 | pAC-U63-gRNA2.1-tubBFP(HA) | 28 | 606778 and 606782 |
Clone frequencies are measured by averaging clone numbers in 10 laterally mounted 3rd instar larvae with pMAGIC gRNA-markers. Only clones in A1 to A7 segments of one side of each larva were counted.
*
Previously published gRNA-markers.
†
3xP3-RFP has been removed from these lines.
‡
Detailed information for each line is available at https://bdsc.indiana.edu/stocks/misc/magic.html.