Skip to main content
. 2002 Oct 1;99(21):13861–13866. doi: 10.1073/pnas.202476699

Figure 1.

Figure 1

Infection of BMDM by wild-type L. monocytogenes, but not an LLO− strain, results in up-regulation of ifnb gene expression. (A) C57BL/6 macrophages were infected at a moi resulting in 1–5 bacteria per cell with L. monocytogenes or treated with E. coli LPS (1 ng/ml), or B. subtilis LTA (1 μg/ml). RNA was isolated from macrophage monolayers at 6 h.p.i. and analyzed by RT-PCR. Actin reactions shown were amplified for 15 cycles; ifnb reactions shown were amplified for 30 cycles. (B) C57BL/6 macrophages were infected as described above and RNA was isolated from macrophage monolayers. Actin reactions shown were amplified for 15 cycles; ifnb reactions shown were amplified for 30 cycles.