Skip to main content
. 2002 Oct 1;99(21):13861–13866. doi: 10.1073/pnas.202476699

Figure 2.

Figure 2

LLO is not required for ifnb gene expression in HeLa cells. (A) Indirect immunofluorescence was performed on HeLa cells at 4 h.p.i. Coverslips were incubated with a polyclonal antibody directed against L. monocytogenes and rhodamine-phalloidin. White arrowheads mark bacteria colocalizing with F-actin; open arrowheads mark bacteria that are not colocalized with F-actin. (B) HeLa cells were infected with L. monocytogenes and treated with cycloheximide at 1.5 h.p.i. RNA was isolated at 6 h.p.i. and subjected to RT-PCR analysis. Actin reactions shown were amplified for 15 cycles; ifnb reactions shown were amplified for 30 cycles.