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NF-κB p65 is translocated to the nucleus when bacterial products are present in the cytosol of macrophages. Indirect immunofluorescence was performed on C3H/HeJ BMDM infected with E. coli expressing OVA with or without LLO by using monoclonal antibody specific for p65 (1:200) and subsequently with a FITC-conjugated secondary goat anti-mouse antibody (1:100).
