Skip to main content
. 2002 Oct 1;99(21):13861–13866. doi: 10.1073/pnas.202476699

Figure 5.

Figure 5

The p38 MAP kinase is phosphorylated when L. monocytogenes is present in the cytosol. (A) C57BL/6 BMDM were infected with wild-type or the LLO− strain of L. monocytogenes or treated with 1 μg/ml LPS as a positive control. Cells were harvested at 1.5 h.p.i. and subjected to SDS/PAGE and Western blot analysis. A phospho-specific antibody was used to detect the phosphorylated form of p38 (Pp38). An antibody against p38 was used to detect total cellular pools of p38 by using the same whole cell lysates. (B) C57BL/6 BMDM were pretreated with p38 inhibitor, SB202190 (SB), or MEK-1 inhibitor, U0126 (U), for 30 min before infection by L. monocytogenes, or treated with LPS (1 ng/ml) or anisomycin (30 μg/ml). RNA was isolated at 6 h.p.i. or 6 h post treatment and analyzed by RT-PCR. Actin reactions shown were amplified for 15 cycles; ifnb reactions shown were amplified for 30 cycles.