Skip to main content
. 2026 Feb 26;17:1741741. doi: 10.3389/fphar.2026.1741741

FIGURE 2.

Panel A presents fluorescence microscopy images of tissue sections stained with DAPI and TUNEL under four conditions: Control, ENG, DOX, and DOX+ENG, showing increased green TUNEL-positive cells in DOX and DOX+ENG. Panel B shows a bar graph of the apoptosis index (%) with higher values in DOX and DOX+ENG groups. Panel C displays Western blot bands for Beclin-1, LC3B, Bax, Bcl-2, and β-actin across the four groups. Panels D, E, and F present bar graphs quantifying the ratios Bcl-2/Bax, Beclin-1/β-actin, and LC3B-II/β-actin, respectively, showing significant group differences indicated by asterisks.

ENG improve DOX-induced apoptosis and autophagy in mice. (A,B) Representative images and quantitative analysis of TUNEL staining of mice myocardium (n = 5). (C–F) Western blotting results of Beclin-1, LC3B, Bax and Bcl-2 in mice cardiac tissue and quantitative analysis (n = 5). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.