Skip to main content
. 2026 Feb 26;17:1741741. doi: 10.3389/fphar.2026.1741741

FIGURE 4.

Figure containing multiple panels evaluating the effects of engeletin and doxorubicin on cell viability, gene and protein expression, and apoptosis in cell culture. Panel A presents a line graph showing cell viability decreasing with increasing engeletin concentration. Panels B, C, E, G, I, and J display bar graphs of mRNA and protein expression levels, and apoptosis indices with statistical significance marked. Panels D and H show western blot images for Bax, Bcl-2, ANP, BNP, and loading control β-actin. Panel F provides fluorescent microscopy images of cells stained with DAPI and TUNEL, comparing four experimental groups: Control, Eng, DOX, and DOX+ENG.

ENG attenuated DOX-induced cardiomyocyte apoptosis. (A) Relative cell viability of ENG. (B,C) Relative cardiomyocyte mRNA levels of Bcl-2 and Bax (n = 5). (D,E) Western blotting results of Bax and Bcl-2 in cardiomyocytes and quantitative analysis (n = 6). (F,G) Representative images and quantitative analysis of TUNEL staining of cardiomyocytes (n = 6). (H–J) Western blotting results of ANP and BNP in cardiomyocytes and quantitative analysis (n = 6). Statistical analysis was performed using one-way ANOVA, followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.