Figure 1.

Northern blot analysis and RT-PCR. (A) Northern blot of canine cerebral cortex poly(A) RNA (1, 5.0 μg; 2, 2.5 μg) probed with (1) ³²P-labeled canine COX-1 cDNA fragment and (2) ³²P-labeled canine antisense oligonucleotide to intron 1. (B) PCR amplification of PCOX-1 in canine cerebral cortex. Lane 1, ethidium bromide-stained gel of amplified products corresponding to PCOX-1a containing intron 1 (upper band) and PCOX-1b (lower band) lacking intron 1; lane 2, Southern blot of the amplified products probed with antisense oligonucleotide to intron 1; lane 3, Southern blot using COX-3 cDNA as probe. (C) Human Multiple Tissue Northern blots (MTN) probed with a ³²P-labeled human antisense oligonucleotide to intron 1 (HCI). The ≈5.2-kb mRNA was detected in blots 1–3 (adult tissues), and 4 (fetal tissues). Am, amygdala; B, brain; C, cerebellum: Cc, cerebral cortex; Fl, frontal lobe; Hi, hippocampus; Ht, heart; K, kidney; Lu, lung; Li, liver; M, skeletal muscle; Md, medulla; Cn, caudate nucleus; Op, occipital pole; P, placenta; Pn, pancreas; Pu, putamen; Sc, spinal cord; Th, thalamus; Tl, temporal lobe; Co, corpus callosum.