Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Mutant and WT BchI were passed through a Superose 12 gel-filtration column in the absence of ATP (○). When 1 mM ATP was included in the buffer, the proteins interacted and a shift toward an increase in size occurred (●). Elution profile of standard proteins: ribonuclease A, 13.7-kDa fraction 19; chymotrypsinogen A, 25-kDa fraction 15; ovalbumin, 43-kDa fraction 11; BSA, 67-kDa fractions 9–10; void, 2,000-kDa fraction 2.
