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. 2005 Nov 9;5:31. doi: 10.1186/1475-2867-5-31

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Copyright © 2005 Abdullah et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpressed aurora B is not colocalized with phosphorylated myosin II regulatory light chain in interphase NRK cells. An NRK cell overexpressing aurora B-GFP was stained with antibodies that specifically recognised myosin II regulatory light chain phosphorylated at Ser19 and then examined the subcelluar localization of aurora B-GFP (a) and phopshorylated myosin II regulatory light chain (c) by confocal laser microscopy. Corresponding phase and merged images (green; aurora B-GFP, red; phosphorylated myosin II regulatory light chain) are shown in panels b and d, respectively. Overexpressed aurora B is associated with the cortex (a and d, arrows), while phosphorylated myosin II regulatory light chain is enriched in the cell periphery (c, and d, arrowheads). Bar, 10 μm.