Figure 4.

Myc expression and Myc-driven gene expression changes in iMyc^Eμ-1 cells. A, expression of Myc in iMyc^Eμ-1 cells, LBL, and normal controls. The average gene expression was calculated and plotted according to the microarray measurements shown in Figure 3. B, RT-PCR of Myc, Myc^His and Aktb mRNA levels (top) and Western blotting of Myc protein (bottom) in normal B cells (lane 1), LBL from an iMyc^Eμ mouse (lane 2), and the iMyc^Eμ-1 cell line (lane 3; SM, size marker). C, real-time qPCR analysis of Myc mRNA levels in iMyc^Eμ-1 and LBL cells. Mean values and standard deviations based on three independent iMyc^Eμ-1 and five LBL samples are shown. D, expression of proliferation signature genes in iMyc^Eμ samples and normal controls. The expression of genes that fall in the proliferation signature defined in Figure 3 was averaged for each cell population and plotted. E, differentially expressed Myc targets in iMyc^Eμ-1 cells (left column) and LBL (right column) compared to normal resting B cells. Relative gene expression levels are depicted according to the color scale at the bottom. Gene designations and names are listed to the right.