FIG. 2.

Fluorescence histogram overlays of B. lactis DSM 10140 (A) and B. adolescentis DSM 20083 (B) stained with 1 μM DiBAC₄(3) for 4 min at 37°C. The x axis represents the corrected DiBAC₄(3) fluorescence for the cell size, obtained by calculating the log ratio of the green fluorescence of DiBAC₄(3) to the side scatter intensity. Results are shown for control untreated cells (a), for cells exposed to 0.05% (b), 0.1% (c), 0.2% (d), or 0.25% (e) dBS, and for cells that were heat treated at 70°C for 30 min (f).