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. 2002 Nov;68(11):5718–5727. doi: 10.1128/AEM.68.11.5718-5727.2002

FIG. 5.

FIG. 5.

Western blot analysis of fungal protein extracts using affinity-purified OmtA PAb. (A) Analysis of SU1 crude protein extracts isolated at three time points (24, 48, and 72 h) from fungal cultures grown in YES liquid medium. The approximate mass of the primary signal (45 kDa) is shown to the right of the blots in panels A and B and to the left of the blot in panels C and D. (B) Analysis of protein extracts from time-fractionated colonies of SU1 grown on PDA medium. Seventy-two-hour-old colonies were fractionated into three concentric rings based on area covered at three time points: S1, 48 to 72 h; S2, 24 to 48 h; S3, 0 to 24 h. (C) Analysis of protein extracts from time-fractionated colonies of CS10 and LW1432 grown on YES agar medium. Lane STD contains molecular mass standards; the masses of standards are shown to the right of the panel. (D) Analysis of protein extracts from time-fractionated colonies of SU1 and AFS10 grown on YES agar medium.