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. 2002 Nov;68(11):5718–5727. doi: 10.1128/AEM.68.11.5718-5727.2002

FIG. 6.

FIG. 6.

OmtA protein localization in time-fractionated colonies of A. parasiticus SU1, AFS10 (afR knockout), CS10, and LW1432 (omtA knockout) grown on YES agar for 72 h. Paraffin-embedded fungal sections were immunolabeled with affinity-purified OmtA PAb (20 μg/ml) followed by Alexa 488-conjugated goat anti-rabbit IgG. (A) Fluorescence images of SU1 (S1, S2, and S3) and AFS10 (R1, R2, and R3). (B) Fluorescence images of CS10 (C1, C2, and C3) and LW1432 (L1, L2, and L3). All colony fractions were analyzed under the same instrument settings. Bar = 50 μm.